Figure 3.

TCS radiosensitized SUNE-1 cells via downregulating the phosphoinositide 3-kinase signaling pathway. Cells were treated with 0, 10 or 20 µM TCS and/or exposed to 4 Gy radiation with dimethylsulfoxide as the control. (A) The expression of p-AKT, AKT, p-ERK, ERK and cleaved caspase-3 was assessed using western blotting. GAPDH expression was used as a loading control. (B-D) The semi-quantification of proteins concentrations were tested using with ImageJ software. The results are presented as the mean ± SD of three independent experiments. *P<0.05 and **P<0.01 vs. control. ^#P<0.05 and ^##P<0.01 vs. radiation alone. TCS, trichosanthin; p, phosphorylated; AKT, protein kinase B; ERK, extracellular signal-regulated kinase.