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Levels of TNF-α, IL-1β and IL-6 in children with or without fractured ankle. In order to determine the increase in inflammatory response in fractured children, the level of TNF-α, IL-1β and IL-6 was detected using RT-qPCR and western blot assay. The mRNA (A) and protein (B) levels of TNF-α, IL-1β and IL-6 in children with or without fractured ankle were detected by RT-qPCR and ELISA, respectively. **P<0.01 vs. Control.Control, healthy children; Patient, children with fractured ankle.
