FIG 11.
Tac1 and Znc1 regulate gene induction by FOH in C. dubliniensis. (A) RT-qPCR analysis comparing FOH induction of CDR1, CDR2, and orf19.320 orthologs in C. albicans and C. dubliniensis. A wild-type C. albicans strain (yLM660) and a C. dubliniensis isolate (CD57) were treated with 25 μM FNZ and 50 μM FOH in YPD culture for the indicated amount of time before collection for RNA extraction. Expression of each pair of orthologs was measured by pan-primers and compared across species by setting the basal expression in the C. albicans strain to 1 (relative expression; upper). Gene fold induction in each species is shown in the lower panels by setting gene basal expression in yLM660 and CD57 individually to 1. (B) LacZ reporter activation by CdTac1 under FNZ and FOH treatment conditions. C. albicans one-hybrid strains expressing LexA-CdTac1131–989 (yLM766) or LexA-CaTac1130–980 (yLM568) fusion proteins were treated with 25 μM FNZ or 50 μM FOH for 2 h and measured for β-galactosidase activity. (C) RT-qPCR analysis showing the effect of tac1 deletion and tac1 znc1 double deletion on FNZ and FOH induction of CDR1, CDR2, and Cd36.83180 in C. dubliniensis. CD57 and its tac1Δ/Δ (yLM764) and tac1Δ/Δ znc1Δ/Δ (yLM765) derivatives were treated with 25 μM FNZ and 50 μM FOH. Basal expression of each gene in CD57 was individually set to 1.