FIG 6.

Mediator requirement for, and recruitment by, Tac1- and Znc1-dependent induction of CDR1 by farnesol. (A) RT-qPCR analysis of CDR1 mRNA expression in a wild-type strain (yLM167) and a med3 null strain (yLM232) after treatment with 50 μM FOH. FNZ (25 μM) induction (30) was performed as a reference. (B) Anti-HA ChIP analysis of Mediator occupancy at the CDR1 promoter in wild-type (yLM695), tac1Δ/Δ (yLM696), znc1Δ/Δ (yLM697), and tac1Δ/Δ znc1Δ/Δ (yLM698) strains expressing C-terminally 3×HA-tagged Med17 treated with 50 μM FOH. Percent recovery of input (Input%) at the 1-up region in a ChIP product obtained from a strain with native MED17 (WT/WT; yLM660) was set to 1.