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. 2018 Oct 24;62(11):e00968-18. doi: 10.1128/AAC.00968-18

FIG 7.

FIG 7

Influence of Mrr2 on FOH and 1-DD target gene expression. (A) RT-qPCR analysis of CDR1 mRNA expression in a wild-type strain (yLM660) and tac1Δ/Δ znc1Δ/Δ strains (yLM701 to yLM704) generated from an otherwise wild-type, mrr2Δ/Δ, stb5Δ/Δ, or cta4Δ/Δ background and treated with FOH (50 μM) and 1-DD (50 μM). CDR1 expression, in the absence of treatment, in yLM660 was set to 1. (B) RT-qPCR analysis of CDR1 mRNA expression in wild-type (yLM660) and mrr2 null (yLM662) strains treated with FNZ (25 μM), FOH (50 μM), or 1-DD (50 μM). CDR1 expression level, in the absence of treatment, in the wild-type strain was set to 1. (C) Anti-HA immunoblot analysis of C-terminally 3×HA-tagged CDR1 protein expression in MRR2 wild-type strains (yLM665 and yLM705) and deletion mutants (yLM707 and yLM706) in the presence and absence of TAC1 and ZNC1 and treatment with FNZ (25 μM) or FOH (50 μM). Blot images acquired at different exposures are presented with a Coomassie blue stain (CBS) as a loading control.