TABLE 1.
Relative colony radius of C. albicans strains grown on farnesol-containing YPD agara
| Strainb | Vehicle | [FOH] (μM) |
|||
|---|---|---|---|---|---|
| 25 | 50 | 100 | 200 | ||
| Wild type | ++++ | +++ | +++ | +++ | +++ |
| cdr1Δ/Δ | ++++ | +++ | + | NG | NG |
| cdr2Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| cdr1Δ/Δ cdr2Δ/Δ | ++++ | ++ | NG | NG | NG |
| tac1Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| tac1Δ/Δ cdr1Δ/Δ | ++++ | +++ | NG | NG | NG |
| znc1Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| znc1Δ/Δ cdr1Δ/Δ | ++++ | +++ | + | NG | NG |
| tac1Δ/Δ znc1Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| tac1Δ/Δ znc1Δ/Δ cdr1Δ/Δ | ++++ | +++ | NG | NG | NG |
| mrr2Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| tac1Δ/Δ znc1Δ/Δ mrr2Δ/Δ | ++++ | +++ | +++ | ++ | ++ |
| TAC1WT | ++++ | +++ | +++ | +++ | +++ |
| TAC1WT cdr1Δ/Δ | ++++ | +++ | ++ | NG | NG |
| TAC1WT cdr1Δ/Δ cdr2Δ/Δ | ++++ | +++ | NG | NG | NG |
| TAC1A736V | ++++ | +++ | +++ | +++ | +++ |
| TAC1A736V cdr1Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| TAC1A736V cdr1Δ/Δ cdr2Δ/Δ | ++++ | +++ | NG | NG | NG |
| TAC1N977D | ++++ | +++ | +++ | +++ | +++ |
| TAC1N977D cdr1Δ/Δ | ++++ | +++ | +++ | +++ | +++ |
| TAC1N977D cdr1Δ/Δ cdr2Δ/Δ | ++++ | +++ | NG | NG | NG |
Each entry in the table represents the colony size observed for a particular combination of strain and treatment. The average radius of colonies formed by a wild-type reference strain (wild type or TAC1WT) on non-FOH-containing plates (vehicle) was set to 100% to normalize colony size of its derivative mutants under each growth condition. Data were symbolized by the following transformation: 90 to 110%, ++++; 70 to 90%, +++; 50 to 70%, ++; 30 to 50%, +. NG, no visible colony formation was seen after 40 h of incubation at 30°C.
Strains tested in this table are marked by their genotypic feature in Table S2. Strains (and their growth data) are divided (horizontal line) with respect to their parental wild-type background (designated wild type or TAC1WT).