FIG 5.

K22 inhibits E protein and dsRNA accumulation in ZIKV-infected cells. Vero cells were pretreated with 30 μM K22 or DMSO for 4 h before infection with ZIKV (MOI = 0.1 TCID₅₀/cell). DMSO-treated and noninfected cells (Mock) were included as controls. The inoculum was removed after 1 h, and the cells were washed with PBS. At 48 h p.i., the cells were fixed and processed for immunofluorescence analysis using antibodies directed against flavivirus E protein and dsRNA as markers of ZIKV replication. Cells were counterstained with phalloidin (actin) or DAPI (nuclei). z-projections of multiple optical sections acquired with a Nikon confocal A1 microscope combined with an Eclipse Ti inverted microscope are shown. Bars, 20 μm.