FIG 6.

Ultrastructural analysis of the K22-mediated alteration of ZIKV replication compartment formation. Vero B4 cells were pretreated with 30 μM K22 for 2 h and infected with ZIKV (MOI = 10 TCID₅₀/cell) for 24 h. The inoculum was removed at 1 h p.i., and the cells were washed with PBS. DMSO-treated and noninfected cells (mock) were included as controls. The cells were fixed at 24 h p.i. and processed for ultrastructural analyses by TEM of ultrathin sections 90 nm thick. Magnifications of representative areas indicated by squares in the left panels are shown on the right.