TABLE 1.
Susceptibility of bacterial strains to MsbA inhibitors
| Parametera | Strainb | Value for the parameter with:c |
||
|---|---|---|---|---|
| G592 | G913 | G332 | ||
| Biochemical IC50 (nM) | Purified E. coli MsbA | 150 ± 48 | 3.8 ± 0.98 | 2.8 ± 0.75 |
| EC50 (μM) | CFT073 lptD(imp4213) msbAlow | 4.5 ± 2.9 | 0.15 ± 0.045 | 0.11 ± 0.032 |
| CFT073 lptD(imp4213) msbAWT | 8.3 ± 2.7 | 0.27 ± 0.075 | 0.19 ± 0.04 | |
| CFT073 lptD(imp4213) msbAhigh | 40 ± 23 | 1.1 ± 0.40 | 0.46 ± 0.16 | |
| MIC (μg/ml) | CFT073 lptD(imp4213) | 21 ± 16 | 0.24 ± 0.13 | 0.22 ± 0.11 |
| CFT073 ΔtolC | 18 ± 5 | 0.22 ± 0.15 | 0.31 ± 0.22 | |
| CFT073 WT | >41 | 39 ± 11 | 2.8 ± 0 | |
| K. pneumoniae ATCC 700721 | ND | >45 | 2.8 ± 0 | |
| E. cloacae ATCC 13047 | ND | >45 | 2.8 ± 0 | |
a
IC50, 50% inhibitory concentration.
b
Low and high levels of MsbA expression are indicated by msbAlow and msbAhigh, respectively; wild-type expression is indicated as msbAWT.
c
For all samples, n ≥ 3. ND, not determined.