TABLE 7.
PCR primers
| Primer name | Primer sequencea |
|---|---|
| msbA-f2 | AAACGTCTCGAATTCATGCATAACGACAAAGATCTCTC |
| msbA-r2 | AAAATCTAGAtCATTGGCCAAACTGCATT |
| PBAD-msbA-f | AAAAGAGCTCGATGCATAATGTGCCTGTC |
| PBAD-msbA-r | AAAAGAGCTCGTTCACCGACAAACAACAG |
| msbA-KO-noPkan-f | TGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCAGGAGGAATTCATGATTGAACAAGATGGATTGC |
| msbA-KO-kan-r | GGCAGCAATAGCCGCCACAAAGGGGATTCACCAGACCAGATTTTTTCGATCATATGAATATCCTCCTTAGTTCCTATTC |
| SmR-f | AAAAAAGACTCCCCGTCAGAAGCACACGGTCACACTG |
| SmR-r | AAAAAAAAATCGAGACCCCTGCTTATCCACGGAATCT |
| msbA-f | ATGCTGGTTTTTCATATGCATAACGAC |
| msbA-r | CGGGATCCTCGAGTCATTGGCCAAACTGCATTTTG |
a
Bolded sequence at 3' ends is homologous to template sequence. Additional bolded sequence in msbA-KO-noPkan-f primer introduces an FRT recombinase site.