FIG 2.
nLuc activity in conversion assay with Ookluc depends on fertilization. (a) nLuc activity, expressed as RLU, in Ookluc and Ookluc-MTRAPKO blood stages (t0) or after 24 h of conversion assay. (b) nLuc activity, expressed as RLU, in Ookluc parasites after 24 h of conversion assay in the presence of 5 μM the phospholipase C inhibitor U73122 or its inactive analog U73343. Control, DMSO. (c and d) nLuc activity, expressed as RLU, in Ookluc parasites after 6 h (c) or 24 h (d) of conversion assay in the presence of 100 μM 2-BP, an inhibitor of palmitoylation, or DMSO control, added at time zero or 1 h postactivation (p.a.). All bars show the means + the SD and are representative of four independent experiments.