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. 2018 Oct 24;62(11):e00737-18. doi: 10.1128/AAC.00737-18

FIG 5.

FIG 5

Overview of the metabolic changes in P. brasiliensis in the presence of argentilactone. Proteins interacting with argentilactone and modulated expression in the presence of argentilactone include the following: phosphofructokinase (PFK), pyruvate dehydrogenase (PDH), citrate synthase (CS), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), succinate dehydrogenase (SDH), superoxide dismutase (SOD), peroxiredoxin (PRX1), thioredoxin (TRX), ATP synthase, mannose-1-phosphate guanyltransferase (MPGT), phosphomannomutase (PMM), UDP-N-acetylglucosamine pyrophosphorylase (NAPP), betaine aldehyde dehydrogenase (BAD), glutathione S-transferase (GST), alcohol dehydrogenase 1 (ALD), isocitrate lyase (ICL), malate synthase (MLS), aspartate aminotransferase (AAT), 4-aminobutyrate aminotransferase (ABAT), dehydrogenase (SSD), 1-pyrroline-5-carboxylate dehydrogenase (PYC), 2-methylcitrate dehydratase (2-MCD), 2-methylcitrate synthase (2-MCS), acyl-CoA dehydrogenase (ACD), MFS multidrug transporter (MFS), benzoate 4-monooxygenase cytochrome P450 (BMC), ABC multidrug transporter (ABC), and trichothecene efflux pump (TEP). The letters in parentheses indicate the following: C, argentilactone-interacting proteins revealed by chemoproteomics analysis; P, differentially expressed proteins in the presence of argentilactone (14); and T, differentially expressed transcripts in the presence of argentilactone (16). The underlined proteins are upregulated, and proteins that are not underlined are downregulated during proteomics or transcriptomics analysis. The symbol of an Erlenmeyer flask with a checkmark on it refers to the processes that were validated experimentally to confirm the influence of argentilactone (proteomic, transcriptomic, and chemoproteomics analysis). The red down arrows indicate processes downregulated/inhibited by argentilactone. Red up arrows indicate processes upregulated/induced by argentilactone. Asterisks refer to experimental validation of the inhibition of isocitrate lyase by the natural compound argentilactone (15). Dotted arrows summarize processes that have many steps.