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. 2018 Oct 24;62(11):e01497-18. doi: 10.1128/AAC.01497-18

FIG 5.

FIG 5

Pharmacokinetic properties of NVR 3-778 in PHHs cultured in different HCM. PHHs that were cultured for 5 days in either HCM without BSA/hA1GP (●) or in HCM spiked with 4.3% BSA–0.07% hA1GP (■) or with 8% BSA–0.07% hA1GP (▲) were incubated with 1 μM NVR 3-778 for 4 days, and both cells and supernatant were harvested at 1, 24, 48, and 72 h postincubation. The parent drug was quantified by LC/MS-MS at the indicated time points. (A) Quantification of the parent drug in the cell culture supernatant of PHHs. (B) Cellular concentration normalized for the hepatocyte volume of NVR 3-778 in PHHs. (C) Predicted liver-to-medium ratio of NVR 3-778 at the indicated time points calculated based on the medium and hepatocyte concentrations of NVR 3-778 in PHHs. Hepatocyte concentrations were normalized for the hepatocellularity to predict the corresponding liver concentrations. All data represent the means of three replicates ± SEM.