FIG 4.
(A) Trypanosoma evansi-infected rabbits. The rabbits were infected with 1 × 105 T. evansi parasites/animal. Rabbits showed clinical signs of lacrimation (a), corneal opacity (b), and swelling of external genitalia (c). (B) Antibodies against T. evansi, determined by ELISA in infected and uninfected rabbits. The rabbits of the infected group were infected with 1 × 105 T. evansi parasites/animal. The uninfected rabbits were inoculated with PBS as a negative control. An asterisk indicates statistically significant differences in antibodies against T. evansi in infected (P < 0.001) and uninfected (P < 0.0001) animals. (C, a) Histopathological changes in different tissues of T. evansi-infected rabbits (H&E staining was used; magnification, ×400). (i) Liver; (ii) spleen; (iii) lung; (iv) kidney; (v) brain. (b) Uninfected rabbits. (i) Liver; (ii) spleen; (iii) lung; (iv) kidney.