FIG 5.

Effect of quinapyramine sulfate-loaded chitosan nanoparticles on T. evansi in rabbits. (A) Giemsa-stained blood smear of rabbit experimentally infected with T. evansi. The white arrow shows the parasite T. evansi, and black arrows depict bunches of parasites. (B) Giemsa-stained blood smear of experimentally infected and treated rabbit. Parasite was not detected in blood smear of the infected and treated rabbit. (C, i) Representative PCR image showing detection of T. evansi DNA (lane L, ladder; lanes 1, 2, and 3, DNA from T. evansi-infected rabbit; lane 4, DNA from infection-negative rabbit. (ii) Lane 1, DNA from infection-negative rabbit; lane 2, DNA from T. evansi-infected rabbit; lanes 3 to 11, DNA from infected and treated rabbits. (D) Percent hemoglobin levels. (E) Body weight. (F) Rectal temperature of infected rabbits before and after treatment with QS and ChQS-NPs.