Figure 5.

AC‐miR‐95‐5p‐Exos regulates the expression of HDAC2/8, Col2A1, aggrecan and SOX9 during chondrogenesis. HMSCs were treated with TGF‐β3 to induce chondrogenesis and co‐cultured with AC‐miR‐95‐5p‐Exos or AC‐anti‐miR‐95‐5p‐Exos. (A, I) The expression level of miR‐95‐5p were estimated by qRTPCR, while the expression levels of HDAC2 (B, J, Q), HDAC8 (C, K, Q), SOX9 (D, L, Q), Aggrecan (E, M), COL2A1 (F, N, Q), RUNX2 (G, O, Q) and MMP13 (H, P, Q) were estimated by both qRT‐PCR and western blotting. U6, GAPDH and β‐actin were used as endogenous controls. Data were presented as means ± standard deviation of three samples. *P < 0.05, **P < 0.01, ***P < 0.001