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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Sep 18;123:168–179. doi: 10.1016/j.yjmcc.2018.09.006

Figure 2. Tetrodotoxin-sensitivity ofINaL under AP-clamp.

Figure 2.

Representative tetrodotoxin (TTX)-sensitive current traces under AP-clamp at 2 Hz steady-state pacing. Increasing TTX concentrations (0.1, 0.3, 1, 3, 10, 30 μM) were applied in a cumulative manner. Pipette solution contained 10 mM BAPTA and extracellular Tyrode solution was supplemented with 10 ^M nifedipine. (B) TTX dose-response effect on INaL net charge and INaL peak. Inhibition of INaL was normalized to that obtained with 30 μM TTX in each cell. IC50 values and Hill coefficients were determined by fitting data to the Hill equation, indicated by solid lines.