Extended Data Figure 2: Activity-dependent labeling of sweet and bitter cortical targets in the amygdala.

Shown are activity-dependent labeling of sweet and bitter cortical targets in the amygdala by probing for c-Fos expression¹⁶ in response to optogenetic activation of the sweet and bitter cortical fields. a, Schematic of optogenetic stimulation strategy in bitter cortex for c-Fos induction. b, c-Fos expression in amygdala in response to photostimulation of bitter cortex. The majority of c-Fos positive neurons are localized to CEA. c, c-Fos expression in a control mouse without light stimulation. d,e,f, Photostimulation of sweet cortex induces c-Fos expression in amygdala. Note that CEA, as a major local output of BLA^11,36, also shows strong c-Fos labeling in response to photostimulation. Scale bars, 200 μm. Similar results were obtained in 3 animals for each experiment.