Table 1.
| Kinetic parameters for MutL activated DNA unwinding by UvrD monomers
| [ATP] | 𝑡̅ (s) |
n (steps) |
k (s−1) |
m = L/n
(bp/step) |
r = L/t
(bp/s) |
|---|---|---|---|---|---|
| 5 μM | 1.54 ± 0.05 | 4.1 ± 0.4 | 2.6 ± 0.3 | 4.4 ± 0.4 | 11.7 ± 0.4 |
| 15 μM | 0.78 ± 0.02 | 5.1 ± 0.6 | 6.7 ± 0.9 | 3.5 ± 0.4 | 23.0 ± 0.7 |
| 50 μM | 0.48 ± 0.01 | 5.9 ± 0.7 | 12.2 ± 1.7 | 3.1 ± 0.4 | 37.6 ± 1.1 |
Best fit parameters (best fit ± s.e.) determined from maximum-likelihood estimation analysis (see Methods) of the data in Fig. 1c to Supplementary Scheme 1 (Eq. 1)