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. Author manuscript; available in PMC: 2019 Oct 19.
Published in final edited form as: J Mol Biol. 2018 Aug 30;430(21):4260–4274. doi: 10.1016/j.jmb.2018.08.022

Table 2.

| Kinetic parameters for DNA unwinding by UvrD alone and UvrD-MutL complexes

Sample m
(bp/step)		 kobs
(s−1)		 kc
(s−1)		 mkobs
(bp/s)		 P ⟨Nbp⟩
(bp)
3’-(dT)20 + UvrD 1.0 ± 0.2 78 ± 15 4.6 ± 0.1 80 ± 30 0.935 ± 0.012 15 ± 3
3’-(dT)20 + UvrD + MutL 2.32 ± 0.05 29.5 ± 0.6 3.95 ± 0.02 68 ± 3 0.977 ± 0.002 42 ± 4
3’-(dT)14 + UvrD + MutL 2.90 ± 0.05 26.9 ± 0.5 4.54 ± 0.02 78 ± 3 0.968 ± 0.001 31 ± 1

Best fit parameters (best fit ± s.e.) determined from non-linear least-squares analysis (see Methods) of the data in Fig. 4a-c to Supplementary Scheme 2 (Eq. 3)