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. Author manuscript; available in PMC: 2018 Nov 13.
Published in final edited form as: Integr Biol (Camb). 2017 Nov 13;9(11):857–867. doi: 10.1039/c7ib00146k

Figure 1. Quantification of IFN induced gene expression in single HepG2 cells.

Figure 1.

(A) The schematics of single-molecule fluorescence in-situ hybridization (smFISH) and simultaneous imaging of RIG-I, MXA, and MDA5 mRNA in individual HepG2 cells treated with IFN-β for 6 hours. (B) Images of RIG-I and housekeeping gene ALAS mRNA in control and IFN-β treated (6 hours) HepG2 cells. (C) Kinetics of RIG-I mRNA expression post IFN-β treatment. Transcript numbers (mean ± SD) are obtained from 10 to 20 cells at each time point.