Table 2.
Number of transcripts detected by Nanostring nCounter gene expression system in control, IFN-β treated (2 hours), and SeV infected (2, 3, 6, and 9 hours) HepG2 cells. Number of transcripts is normalized to housekeeping gene GAPDH. Green rows denote viral genes, blue rows cytokines, dark gray rows early ISGs.
| Basal | IFN-β St. | 2 hpi | 3 hpi | 6 hpi | 9 hpi | |
|---|---|---|---|---|---|---|
| L gene | 4 | 15 | 240 | 466 | 1485 | 12720 |
| N gene | 1 | 13 | 1511 | 3179 | 13252 | 101475 |
| IFNA1 | 1 | 14 (14) | 1.5 (1.5) | 1 (1) | 2 (2) | 7 (7) |
| IFNA4 | 7 | 14 (2) | 6 (0.9) | 3 (0.4) | 9 (1.3) | 19 (3) |
| IFN-β | 6 | 6 (1) | 3 (0.5) | 3 (0.5) | 50 (8) | 2613 (436) |
| TNF-α | 35 | 66 (2) | 49 (1.4) | 56 (1.6) | 200 (6) | 3248 (93) |
| DDx60 | 1 | 514 (514) | 14 (14) | 17 (17) | 122 (122) | 2456 (2456) |
| IFIT1 | 62 | 17289 (278) | 107 (1.7) | 455 (7) | 3119 (50) | 41351 (667) |
| ISG15 | 1923 | 48622 (25) | 2022 (1.1) | 2862 (1.5) | 9524 (5) | 113474 (59) |
| LGP2 | 94 | 558 (6) | 107 (1.1) | 78 (0.8) | 187 (2) | 1412 (15) |
| MDA5 | 420 | 4527 (11) | 402 (1) | 509 (1.2) | 1051 (3) | 11027 (26) |
| OasL | 12 | 2157 (180) | 27 (2) | 119 (10) | 1199 (100) | 22884 (1902) |
| RIG-I | 306 | 4890 (16) | 215 (0.7) | 303 (1) | 613 (2) | 6968 (23) |
| Viperin | 1 | 3942 (3942) | 2 (1) | 6 (6) | 107 (107) | 2955 (2955) |
| IRF1 | 221 | 1994 (9) | 264 (1.2) | 238 (1.1) | 306 (1.4) | 1256 (6) |
| IRF7 | 2 | 20 (10) | 3 (1.5) | 1 (0.5) | 3 (1.5) | 9 (4.5) |
| IFITM3 | 3898 | 11812 (3) | 4092 (1) | 4595 (1.2) | 4809 (1.2) | 9809 (3) |
| MxA | 279 | 13396 (48) | 291 (1) | 318 (1.1) | 382 (1.4) | 3230 (12) |
| MyD88 | 1648 | 4590 (3) | 1744 (1.1) | 1862 (1.1) | 1711 (1) | 2381 (1.4) |
| Oas1 | 330 | 1457 (4) | 274 (0.8) | 285 (0.9) | 312 (1) | 751 (2) |