Table 2.

Frontal Affinity Chromatography—Mass Spectrometry (FAC— MS) Results for Library A and Library B Screened with Immobilized GPR17^a

Compd	tb(min)	t/tcc × 100
	Library A
UDP	3.20	13.03
13	4.06	16.54
9	4.78	19.47
14	4.83	19.67
12	6.38	25.99
MRS2179	7.10	28.92
5	7.53	3.67
11	8.86	36..9
7	9.22	37.56
8	11.06	45.05
6	12.25	49.90
10	16.80	68.43
Cangrelor	24.55	100.00
4	29.55	120.37
	Library B
UDP	1.50	12.30
15	1.51	12.38
18	1.60	13.11
19	2.42	19.84
16	2.83	23.20
17	3.24	26.56
MRS 2179	3.40	27.87
20	3.58	29.34
21	9.22	75.57
Cangrelor	12.20	100.00

^aThe breakthrough times are from a single raking experiment carried out on the freshly prepared column. Column: GPR17-IAM-I (20.72 milion cells). Breakthrough times of the three reference compounds (UDP, MRS 2179, and cangrelor) are marked in bold. See text for detailed chromatographic conditions

^bt is the breakthrough time of the ligand with the immobilized GPR17.

^ctc represents the breakthrough time of the indicator cangrelor.