Figure 4.

Administration of H11/AIM2 nanoparticles inhibits the tumour growth of 786‐O cell‐xenografted nude mice. The 786‐O cell‐xenografted nude mice were administrated intratumorally H1/pAIM2 or control (50 μg/mouse) on day 7 post‐injection when the tumour reached about 50 mm³ in volume weekly for total 3 times. (A). AIM2 expression was detected by western blot in tumour tissues. (B). The present images of tumour excised from mice treated with H1/pAIM2 or control after 35 d of growth in vivo. (C). Tumour progression of 786‐O cell‐xenograft was evaluated by measurement of tumour volume twice per 7 d from day 0 to day 35. (D). The tumour volumes were measured at day 35 after tumour inoculation. (E). The tumour weights were monitored at day 35 after tumour inoculation. (F). Body weights of mice were detected per 2 d from day 1 to day 35. (G). At day 2 or 7 after the initial administration, the serum were collected, the levels of IL‐6 were determined by ELISA assay. (H) At day 35 after tumour inoculation, the pathology was evaluated by H&E staining of heart or liver tissues. Each experiment was performed independently at least three times and the results of one representative experiment are shown. Data are means ± SD, ***P < 0.001, ns, no significant difference