Figure 2.

Polydatin (PD) protects NPCs from senescence induced by TNF‐α. A, Representative micrographs showed the effects of PD on senescence in NPCs treated with 10 ng/mL TNF‐α for 72 h, which was manifested by the SA‐β‐gal staining assay. Scale bar: 100 μm. B, D, The expression of p53 and p16 was detected by Western blotting. NPCs treated with or without preadministration of PD for 2 h were administrated with 50 ng/mL TNF‐α for another 72 h. C, NPCs were treated with PD or TNF‐α as described above and incubated with EdU (10 μmol/L). Then, NPCs were fixed and stained with the antibody for EdU, and nuclei were stained with DAPI. Scale bar: 10 μm. The values presented are the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, n = 5