Figure 3.

Polydatin (PD) suppresses extracellular matrix degradation induced by TNF‐α in NPCs. NPCs were pretreated with different concentrations of PD before administration with 50 ng/mL TNF‐α for another 72 h. A, B, The expressions of aggrecan were detected by Western blot. C, D, The expression of collagen II (COL II) in NPCs treated as above was detected by immunofluorescence. Scale bar: 50 μm. E‐H, The expression of MMP‐3, MMP‐13 and ADAMTS‐4 was detected by Western blot analysis. Data presented are the means ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs control group; *P < 0.05, **P < 0.01, n = 5