Figure 6.

Knockdown of Nrf2 compromises the antisenescence, anti‐ECM disorder and antimitochondrial dysfunction properties of PD on NPCs. A‐C, Successful knockdown of Nrf2 suppressed the expression level of HO‐1. D, E, The expression of p53 and p16 in NPCs treated with or without pretreatment of PD for 2 h and another administration of TNF‐α for 72 h. F, G, The levels of ECM‐related proteins such as aggrecan, MMP‐13 and MMP‐3 were detected by Western blotting. H‐J, Representative images for MitoSOX and MitoTracker of PD and TNF‐α‐treated NPCs with or without Nrf2 knockdown. Scale bar: 50 μm. Data presented are the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, n = 5