Figure 2.

GCN5 knockdown caused G1 arrest and inhibited DNA synthesis in HPV‐16 E7‐expressing cells. (A) The protein level of GCN5 was measured by Western blot after transfected with siRNAs targeting GCN5 for 48 h. (B) Flow cytometry of cells treated with bleomycin for 36 h after transfected cells with GCN5 siRNA for 24 h and then stained with PI. G1, S and G2 phases are indicated and quantified. (C) Flow cytometry of cells transfected with GCN5 siRNA for 48 h and labelled with 20 nmol/L BrdU for 2 additional hours. Cells were stained with anti‐BrdU antibody, counterstained with 7‐AAD and analysed by flow cytometry. (D) Flow cytometry of cells treated with bleomycin for 36 h after transfected cells with GCN5 siRNA for 24 h. Data from a representative experiment of 3 are shown, *P < 0.05; **P < 0.01