Figure 4.

E2F1‐depended regulation of Cdk1 by GCN5 in E7‐expressing cells. (A) Western blot analysis of cell cycle‐related proteins after transfection with GCN5 siRNAs. (B) Flow cytometry of cells treated with bleomycin for 36 h after transfected cells with E2F1 siRNA for 24 h and then stained with PI. (C) Flow cytometry of cells transfected with E2F1 siRNA for 48 h and labelled with 20 nmol/L BrdU for 2 additional hours. Cells were stained with anti‐BrdU antibody, counterstained with 7‐AAD and analysed by flow cytometry. (D) Flow cytometry of cells treated with bleomycin for 36 h after transfected cells with E2F1 siRNA for 24 h and labelled with 20 nmol/L BrdU for 2 additional hours. (E) Western blot analysis of E2F1 after cells was transfected with pCMV or pCMV‐E2F1 plasmid. (F) Flow cytometry of cells treated with bleomycin for 36 h after transfection with GCN5 siRNA, and pCMV or pCMV‐E2F1 plasmid, and then stained with PI. Data from a representative experiment of 3 are shown, *P < 0.05; **P < 0.01