Figure 4.

The rs3746444 polymorphism reduces target suppression by miR‐499‐5p. A and B, G‐allelic pre‐miR‐499 causes a lesser inhibition of CnAα‐3′UTR and CnAβ‐3′UTR translation activity than the A‐allelic one. HEK293 cells were transfected with the luciferase constructs of CnAα‐3′UTR (A) or CnAβ‐3′UTR (B), along with the plasmids for empty pcDNA3.1, pri‐miR‐499‐A or pri‐miR‐499‐G, respectively. Luciferase activity was measured after 36 hours. pGL3 served as a control. Data are expressed as the mean ± SD of 3 independent experiments. *P < 0.05. C, G‐allelic pre‐miR‐499 suppresses the expression of CnAα and CnAβ at a lesser degree than the A‐allelic one. H9c2 cells were transfected with the plasmids for empty pcDNA3.1, pri‐miR‐499‐A or pir‐miR‐499‐G, respectively. Twenty‐four hours after infection, CnAα and CnAβ protein levels were detected by immunoblot. A representative blot of three independent experiments is shown