Table 3.
Cytokeratin‐18 and vimentin staining profiles in NSCD and SCD surgical resection specimens
| Sample Pair | Cytokeratin‐18 Extra‐epithelial cells positively stained (%) | Vimentin Area positively stained for per slide (%) | ||
|---|---|---|---|---|
| NSCD | SCD | NSCD | SCD | |
| 1 | 0.59 | 1.94 | 0.00 | 5.53 |
| 2 | 0.25 | 0.35 | 7.21 | 10.49 |
| 3 | 0.00 | 0.64 | 12.67 | 27.16 |
| 4 | 0.08 | 0.39 | 4.17 | 6.00 |
| 5 | 0.21 | 0.24 | 4.57 | 12.78 |
| 6 | 0.04 | 0.20 | 9.34 | 27.51 |
| 7 | 0.04 | 0.08 | 2.76 | 27.62 |
| 8 | 0.06 | 0.26 | 10.12 | 24.83 |
| 9 | 0.06 | 0.29 | 8.98 | 14.58 |
| Mean | 0.15 | 0.49 | 6.65 | 17.39 |
| SEM | 0.06 | 0.19 | 1.35 | 3.13 |
| Student's t test | P = 0.043 | P = 0.003 | ||
For cytokeratin‐18 the ImageJ program was used as described to count the total number of cells and number of positively stained cells in 3 randomly selected fields of ×20 magnification. The sum of the numbers of cells for each field was totalled and a percentage of positive staining cells calculated for each sample as shown below. For vimentin, the ImageJ program was used to calculate the total area (in pixels) assessed and the area positively stained for vimentin (in pixels) in three randomly selected crypts and two randomly selected regions of one hundred consecutive surface epithelial cells at ×20 magnification. The latter was expressed as a percentage of the former. Each data point is an average of percentage values across the three crypts and two surface epithelial areas for each slide. The mean percentages are shown for each group (NSCD vs SCD) and the standard error of the mean (SEM) with the P value as calculated using a Student's t test showing statistical significance.