Figure 2.

LINC01287 inhibition decreased hepatocellular carcinoma (HCC) cell proliferation and invasion. A, LINC01287 expression in Huh‐7 cells transduced with control shRNA vector (sh‐ctrl) or LINC01287 shRNA vector (sh‐LINC01287). B, The MTT assay revealed that LINC01287 down‐regulation significantly decreased cell proliferation. C, Colony formation assay demonstrated that oncogenic survival was significantly decreased in sh‐LINC01287 cells when compared with sh‐ctrl cells. D, sh‐LINC01287 cells displayed a significantly higher frequency of cells at the G1 phase and a lower frequency of cells at S phase. E, LINC01287 down‐regulation affected the expression of G1/S phase checkpoint proteins. F, LINC01287 down‐regulation decreased the HCC cell invasion ability, as revealed by the Boyden assay. G, The expression levels of MMP‐2, MMP‐3 and MMP‐9 protein were lower in sh‐LINC01287 cells. H, Expression level of E‐cadherin increased, while expression levels of N‐cadherin and vimentin were decreased in sh‐LINC01287 cells