Figure 3.

Inhibition of pharmorubicin‐induced autophagy down‐regulated cell viability and siRNAs selection. A, MTT assay revealed that the cell survival of chloroquine‐MDA‐MB‐231 and chloroquine‐MCF‐7 was decreased after being treated with pharmorubicin. B, The cell survival of chloroquine‐MDA‐MB‐231/EP1 and chloroquine‐MCF‐7/EPI was down‐regulated after pharmorubicin treatment. C, The mRNA expression level of HO‐1 and Akt was significantly down‐regulated with the transfection of siRNAs. *P < 0.05, **P < 0.01, compared with NC group