Figure 5.

Akt was the upstream signal of HO‐1 inMDA‐MB‐231/EP1 and MCF‐7/EPI cells. A, The decrease in cell survival in si‐Akt group was showed in MTT assay. B, The result of qRT‐PCR revealed that the mRNA expression of HO‐1 in MDA‐MB‐231/EP1 and MCF‐7/EPI cells was down‐regulated after being transfected with si‐Akt. C, Western blot tested the protein expression of p‐Akt, HO‐1, Beclin‐1, LC3‐II/LC3‐I in si‐Akt group. The results showed that the protein expression in si‐Akt group was lower than that in NC group. D, The cell autophagy of MDA‐MB‐231/EP1 and MCF‐7/EPI cells in si‐Akt group was down‐regulated compared with NC group, scale bar: 20 μm. *P < 0.05, **P < 0.01, compared with si‐Akt‐NC, pharmorubicin (‐) or NC group