Figure 5.

Phospho‐ULK1 induced by PEDF is important for FUNDC1‐mediated mitophagy. A, Western bolt for p‐ULK1 (Ser317, 555, 777), ULK1 and p‐FUNDC1 in cardiomyocytes treated with PEDF or PEDF+Go6976 before OGD 4 for hours, n = 6. S317/555/777A mutants were transfected into cardiomyocytes as indicated. *P < 0.05 vs relative normal control, ^# P < 0.05, ^## P < 0.01 vs relative OGD control. (B) Representative images showing LC3 staining in different groups of cardiomyocytes infected with GFP‐RFP‐LC3 adenovirus for 24 hours, bar = 60 μm. Cardiomyocytes were treated with PEDF or PEDF+Go6976before OGD 4 hours, n = 30 from three independent experiments