Table 2.
Real‐time qPCR SYBR green primer sequences
| mRNA | Primer name | Sequence |
|---|---|---|
| ABCB1 30 | ABCB1‐F | AGCAGAGGCCGCTGTTCGTT |
| ABCB1‐R | CCATTCCGACCTCGCGCTCC | |
| ABCC2 | ABCC2‐F | GGGCTGCTTTATTTCTTGAGGG |
| ABCC2‐R | GATGCCTGCTCTTGCTCCTT | |
| ABCG2 30 | ABCG2‐F | TGGAATCCAGAACAGAGCTGGGGT |
| ABCG2‐R | AGAGTTCCACGGCTGAAACACTGC | |
| HLAG 62 | HLAG‐F | TGCTGAGATGGAAGCAGTCTTC |
| HLAG‐R | ACTACAGCTGCAAGGACAACCA | |
| PCNA | PCNA‐F | TTGAAGCACCAAACCAGGAGA |
| PCNA‐R | TGCAAATTCACCAGAAGGCA | |
| GCM1 16 | GCM1‐F | GGCACGACGGACGCTTTAT |
| GCM1‐R | GCTCTTCTTGCCTCAGCTTCTAA | |
| GJA1 29 | GJA1‐F | GGGGGATCCATGGGTGACTCGAGC |
| GJA1‐R | GGGAAGCTTCTAGATCTCCAGGTCATA | |
| ERVW‐1 | ERVW‐1F | AGAAAAGGCCCCAAGAGGTAATAAAG |
| ERVW‐1R | CCTGGAAAGCAGGGCTATTG | |
| ASCT‐2 29 | ASCT‐2F | CCGCTTCTTCAACTCCTTCAA |
| ASCT‐2R | ACCCACATCCTCCATCTCCA | |
| YWHAZ | YWHAZ‐F | ACTTTTGGTACATTGTGGCTTCAA |
| YWHAZ‐R | CCGCCAGGACAAACCAGTAT | |
| TOP1 | TOP1‐F | GATGAACCTGAAGATGATGGC |
| TOP1‐R | TCAGCATCATCCTCATCTCG |
All primers were blasted using NCBI primer blast to ensure specificity to the gene of interest and confirmed to produce a single band of the expected size by PCR analysis.