Table 3.
Antibodies and Conditions
| Antibody | Species P = poly M = mono | Source catalog No. | Dilution | Antigen retrieval | Cell type/protein target |
|---|---|---|---|---|---|
| Cytokeratin 8/18 | Guinea Pig P | Abcamab194130 | 1:100 | Heat: Sodium Citrate buffer pH6 |
All epithelial cells CT, ST and EVT |
| HLA‐G (4H84) | Mouse M | Exbio 11‐499‐c100 | 1:300 | Heat: Sodium Citrate buffer pH6 | EVT cell specific |
| Anti‐MDR1 (D11) | Mouse M | Santa Cruz SC‐55510 | 1:250 | Heat: Sodium Citrate buffer pH6 | P‐gp protein |
| Anti‐P‐gp EPR 10364.57 | Rabbit M | Abcam ab170904 | 1:1000 | Western Blot | P‐gp protein |
| Syncytin‐1 | Rabbit P | Santa Cruz SC‐50369 | 1:100 | Heat: Sodium Citrate buffer pH6 | ST marker |
| α‐hCG INN hFSH‐132 | Mouse M | Acc. Chem. and Sci. Corp. YSRTMCA1026 | 1:200 | Heat: Sodium Citrate buffer pH6 | Expressed by ST and found at junction of CT and ST |
| α‐1 integrin (ITGA1) | Rabbit P | Chemicon AB1934 | 1:500 | Proteinase K, 10 mg/mL 37°C, 10 min | Invasive EVT |
| c‐erbB2 (HER2) | Rabbit M | DAKO A0485 | 1:300 | Triton 0.01%, 10 min | Invasive EVT and metastatic epithelial breast cancer cells |
| Cx43 | Rabbit P | Invitrogen | 1:100 | Triton 0.01%, 10 min | Junction of CT and ST |
All antibodies used in the study were optimized for antigen retrieval and dilution.