Table 1.

Steady‐State Parameters Measured Before and Following Myk461 Incubation in WT and KO Myocardium

Group	pCa50	nH	Fmax (mN/mm2)	Fmin (mN/mm2)
WT
Pre‐Myk461	5.99±0.02	2.01±0.18	12.52±1.63	1.00±0.09
0.5 μmol/L Myk461	5.97±0.02	3.04±0.09a	11.10±1.41	0.82±0.09
1.0 μmol/L Myk461	5.95±0.01	3.24±0.07a	9.42±0.97	0.78±0.09
KO
Pre‐Myk461	6.05±0.02b	2.24±0.21	12.21±1.29	1.13±0.17
0.5 μmol/L Myk461	6.02±0.01b	2.66±0.12	10.41±1.28	1.29±0.15
1.0 μmol/L Myk461	6.01±0.02b	2.91±0.25a	11.10±1.70	1.38±0.18b

Values are expressed as mean±SE of mean. Twelve skinned myocardial preparations (3 fibers each from 4 hearts) were used for both groups. Linear mixed model was used for data analysis with Tukey post hoc tests for multiple comparisons between groups of data. F_max indicates Ca²⁺‐activated maximal force measured at pCa 4.5; F_min, Ca²⁺‐independent force measured at pCa 9.0; KO, knockout; n_H, cooperativity of force production; pCa₅₀, myofilament Ca²⁺ sensitivity; WT, wild‐type.

^aSignificantly different vs the corresponding pre‐Myk461 group (P<0.05).

^bSignificantly different vs the corresponding WT group (P<0.05).