Table 1.
Antibodies for Western Blot Analysis
| Kinase/Phosphorylation Site | Manufacturer | Order No. | Antibody Dilution | Source | Clonality |
|---|---|---|---|---|---|
| Phospho‐AKT1/2/3ser473 | Cell Signaling | #9271 | 1:500 | Rabbit | Polyclonal |
| AKT1/2/3 | Cell Signaling | #9272 | 1:500 | Rabbit | Polyclonal |
| Phospho‐eNOSser1177 | Cell Signaling | #9571 | 1:500 | Rabbit | Polyclonal |
| eNOS | BD Biosciences | #610296 | 1:500 | Mouse | Polyclonal |
| Phospho‐ERK1thr202/tyr204Phospho‐ERK2thr185/tyr187 | Cell Signaling | #9101 | 1:1000 | Rabbit | Polyclonal |
| ERK1/2 | Cell Signaling | #9102 | 1:1000 | Rabbit | Polyclonal |
| GAPDH | HyTest | #5G4 | 1:500 | Mouse | Monoclonal |
| Phospho‐GSK‐3βser9 | Cell Signaling | #9336 | 1:500 | Rabbit | Polyclonal |
| GSK‐3β | BD Biosciences | #610202 | 1:500 | Mouse | Polyclonal |
| Phospho‐p38 MAPKthr180/tyr182 | Cell Signaling | #9211 | 1:750 | Rabbit | Polyclonal |
| p38 MAPK | Cell Signaling | #9212 | 1:1000 | Rabbit | Polyclonal |
| PKCε | Santa Cruz Biotechnology | #sc‐214 | 1:500 | Rabbit | Polyclonal |
| Phospho‐STAT3tyr705 | Cell Signaling | #9138 | 1:750 | Mouse | Monoclonal |
| STAT3 | Cell Signaling | #12640 | 1:500 | Rabbit | Monoclonal |
| Phospho‐STAT5tyr694 | Cell Signaling | #9359 | 1:250 | Rabbit | Monoclonal |
| STAT5 | Cell Signaling | #9363 | 1:500 | Rabbit | Polyclonal |
AKT indicates protein kinase B; eNOS, endothelial nitric oxide synthase; ERK1/2, extracellular signal‐regulated kinase 1/2; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; GSK3β, glycogen synthase kinase 3β; MAPK, p38 mitogen‐activated protein kinase; PKCε, protein kinase C epsilon; STAT, signal transducer and activator of transcription.