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. 2018 Jul 24;7(15):e009501. doi: 10.1161/JAHA.118.009501

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© 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Dark‐ and light‐phase analysis of urine and water/food intake. Rats were individually housed in metabolic cages for 24 hours. Urine was collected during the dark‐phase (active) and during the lights‐phase (resting) period. Data are expressed as mean±SEM for (A) water intake, (B) food intake, (C) urine volume, (D) creatinine excretion, (E) Na⁺ excretion, (F) K⁺ excretion, (G) creatinine clearance, (H) urine osmolality, and (I) osmolyte excretion. *P<0.05; **P<0.001 vs control, ANOVA for repeated‐measures post hoc Tukey test. NOS indicates nitric oxide synthase.