Table 5.

Mammalian viral replication of the novel reassortant H6N1 avian influenza viruses isolated from poultry in Eastern China, ZJ-1664 and ZJ-1667

Virus	No. of survivors/no. tested	HI titer (log2)	Virus replication in experimentally infected mice Virus titers in organs of mice (log10 EID50/ml)
			Tissue	3 day	6 day	9 day
ZJ-1664	6/6	6.33 ± 0.52	Lung	2.67 ± 0.57	3.67 ± 0.57	2.33 ± 0.57
			Brain	0	0	0
			Heart	0	1.33 ± 0.57	0
			Liver	0	0	0
			Kidney	0	0	0
			Spleen	0	0	0
ZJ-1667	6/6	5.83 ± 0.75	Lung	2.33 ± 0.57	2.67 ± 0.57	1.33 ± 0.57
			Brain	0	0	0
			Heart	0	0	0
			Liver	0	0	0
			Kidney	0	0	0
			Spleen	0	0	0

Fifteen mice were intranasaly inoculated with 10^6.0 EID50 of the H6N1 virus in a 0.05-ml volume of PBS. Six mice were observed for survival for 14 days. The remaining nine mice from each experimental group were exsanguinated on day 3, 6 and 9, and lungs, brains, hearts, kidneys, spleens, and livers were collected for virus titration in embryonated chicken eggs by the method of Reed and Muench. Sera were harvested at 14 days after infection from the six remaining mice. Seroconversion was confirmed by the hemagglutination inhibition (HI) test. Data represent mean ± SD