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Overview of data acquisition workflow. Proteins were extracted from mitochondria of myoblast and myotube cells. GELFrEE size-based prefractionation was performed in the case of integrated top-down (MS1/MS2) and intact-mass (MS1-only) measurements used for proteoform identifications, whereas no such pre-fractionation was employed for MS1-only measurements used for quantification. Lightning bolts denote fragmentation processes.
