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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: J Comp Neurol. 2017 Nov 11;526(3):467–479. doi: 10.1002/cne.24345

Figure 5: Quantification of double-labeled AII amacrine cells.

Figure 5:

(a) Wholemount retinas stained for NFIA and DNER allow for the identification of the AII amacrine cell population, as these cells have nuclei that are NFIA+ residing at the inner margin of the INL and have a DNER+ stalk extending into the IPL (yellow asterisks). NFIA+ but DNER- populations are present (magenta daggers), as are DNER+ but NFIA- populations (magenta section symbols). (b) The distribution of DNER+/NFIA+ AII amacrine cells, identified in (a), illustrates the method of counting cells to estimate total cell number. (c) Estimates of the total number of AII amacrine cells, derived from B6/J retinas in which Prox1+ amacrine cells were counted (Keeley et al., 2014), were strikingly similar to estimates from B6/J retinas in which DNER+/NFIA+ amacrine cells were counted. n = number of retinas. Scale bars = 25 μm.