Table 1:
Antigen | Abbreviation | Type | Immunogen | Supplier / Cat# / RRID | Dilution | Structures Labeled in Mouse Retina |
---|---|---|---|---|---|---|
Delta/Notch-like EGF-related Receptor | Dner | Goat Polyclonal | Mouse Dner, peptide mapping to amino acids 26–637. | R&D
Systems AF2254 RRID:AB_355202 |
1:250 | Determined in this study |
Nuclear Factor 1A-type | Nfia | Rabbit Polyclonal | Human NFIA, peptide mapping to amino acids 301–401. | Atlas
Antibodies HPA006111 RRID:AB_1854422 |
1:500 | Determined in this study |
Prospero Homeobox 1 | Prox1 | Rabbit Polyclonal | Mouse Prox1, peptide mapping to amino acids 723–737. | Covance PRB-238C RRID:AB_10064230 |
1:1,000 | AII amacrine cells Bipolar cells Horizontal cells |
Tyrosine Hydroxylase | TH | Sheep Polyclonal | Rat TH, purified from a pheochromocytoma. | Millipore AB1542 RRID:AB_90755 |
1:1,000 | Dopaminergic amacrine cells |
Calbindin 1 | Calb | Rabbit Polyclonal | Bovine Calb D-28K, purified from cerebellum. | Millipore PC253L RRID:AB_213554 |
1:10,000 | Horizontal cells Amacrine cells Ganglion cells |
Vesicular Glutamate Transporter 3 | VGluT3 | Goat Polyclonal | Human VGLUT3, peptide mapping near the N-terminus. | Santa Cruz
Biotechnology SC26031 RRID:AB_2187701 |
1:500 | VGluT3+ amacrine cells |
Protein Kinase C | PKC | Mouse Monoclonal | Human PKCγ, peptide mapping to amino acids 499–697. | Millipore 05–983 RRID:AB_568862 |
1:500 | Rod bipolar cells Amacrine cells |
Synaptotagmin 2 | Syt2 | Mouse Monoclonal | Homogenized whole zebrafish. | ZIRC ZDB-ATB-081002–25 RRID:AB_10013783 |
1:100 | Type 2 bipolar cells Type 6 bipolar cells |
Glutamine Synthetase | GS | Mouse Monoclonal | Human GS, peptide mapping to amino acids 1–373. | BD
Biosciences 610517 RRID:AB_397879 |
1:500 | Müller glia |
Glial Fibrillary Acidic Protein | GFAP | Mouse Monoclonal pre-conjugated to Cy3 | Pig GFAP, purified from spinal cord. | Sigma C9205 RRID:AB_476889 |
1:500 | Retinal astrocytes |
Choline Acetyltransferase | ChAT | Goat Polyclonal | Human ChAT, purified from placenta. | Millipore AB144P RRID:AB_2079751 |
1:250 | Cholinergic amacrine cells |