Table 2:
Secondary antibodies used in this study.
| Antigen | Type | Fluorophore | Supplier / Cat# / RRID | Dilution |
|---|---|---|---|---|
| Mouse IgG | Donkey Polyclonal | DyLight488 | Thermo Fisher
Scientific SA5–10166RRID:AB_2556746 |
1:200 |
| Mouse IgG | Donkey Polyclonal | AlexaFluor594 | Jackson
ImmunoResearch 715–585-150 RRID:AB_2340854 |
1:200 |
| Mouse IgG | Donkey Polyclonal | Cy5 | Jackson
ImmunoResearch 715–175-150 RRID:AB_2340819 |
1:200 |
| Goat IgG | Donkey Polyclonal | AlexaFluor488 | Jackson
ImmunoResearch 705–545-147 RRID:AB_2336933 |
1:200 |
| Goat IgG | Donkey Polyclonal | AlexaFluor546 | Thermo Fisher
Scientific A-11056 RRID:AB_142628 |
1:200 |
| Goat IgG | Donkey Polyclonal | AlexaFluor647 | Jackson
ImmunoResearch 705–605-147 RRID:AB_2340437 |
1:200 |
| Rabbit IgG | Donkey Polyclonal | AlexaFluor488 | Jackson
ImmunoResearch 711–545-152 RRID:AB_2313584 |
1:200 |
| Rabbit IgG | Donkey Polyclonal | AlexaFluor546 | Thermo Fisher
Scientific A-10040 RRID:AB_2534016 |
1:200 |
| Rabbit IgG | Donkey Polyclonal | AlexaFluor647 | Jackson
ImmunoResearch 711–605-152 RRID:AB_2492288 |
1:200 |
| Sheep IgG | Donkey Polyclonal | AlexaFluor488 | Thermo Fisher
Scientific A-11015 RRID:AB_141362 |
1:200 |