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. 2018 Oct 17;24:7424–7430. doi: 10.12659/MSM.910601

Figure 3.

Figure 3

Effects of ILK expression downregulation on NF-κB activity. (A) Effects of ILK siRNA treatment on NF-κB activity in EMT cell model. (B) Effects of ILK siRNA treatment on cell migration. (C) Effects of ILK siRNA treatment on E-cadherin expression. (D) Effects of ILK siRNA treatment on α-SMA expression. Each experiment was independently repeated 3 times. Control group was TGF-β-induced transdifferentiated cells. ** Compared with control group, p<0.01; RLU, relative light unit. Similar results were found after treatment with QLT0267. Compared with control cells, treatment with QLT0267 significantly reduced the NF-κB activity (Figure 4A, p<0.01), inhibited cell migration (Figure 4B, p<0.01), promoted the expression of E-cadherin (Figure 4C, p<0.01), and reduced the expression of α-SMA (Figure 4D, p<0.01). Those data suggest that expression and activity of ILK are essential for the activity of NF-κB as well as migration and EMT of HLEC-h3 cells. Each experiment was independently repeated 3 times.