Table 1.
Twenty polymorphic EST‐SSR markers developed for Carex angustisquama
| Locus | Primer sequences (5′–3′) | Repeat motif | Allele size range (bp) | BLASTX top hit description | E‐value | GenBank accession no. |
|---|---|---|---|---|---|---|
| Cang_681 | F: TGTGGAATTGTGAGCGGAGCTTATTGGCCGCATGAAC | (AG)19 | 245–251 | B‐box zinc finger protein 22 [Ananas comosus] | 8.00E‐77 | FX986011 |
| R: GTTTCTTCCAACCGGATAAAGCTGCG | ||||||
| Cang_1267 | F: TGTGGAATTGTGAGCGGTAATGTGGGTCCCGGTACTG | (AGC)9 | 209–221 | PREDICTED: ATP‐dependent helicase BRM [Oryza brachyantha] | 0.0 | FX985999 |
| R: GTTTCTTCGTGAAACCGAAACCTGGTC | ||||||
| Cang_1881 | F: TGTGGAATTGTGAGCGGTGTGGATGACGTGGCATTTG | (AT)11 | 316–334 | Hypothetical protein GQ55_7G126900 [Panicum hallii var. hallii] | 9.00E‐149 | FX986003 |
| R: GTTTCTTTACAGCACAACATAGCCCTC | ||||||
| Cang_2073 | F: CTATAGGGCACGCGTGGTCAGTGCAGCCGAGATTCTTG | (AAG)10 | 466–475 | Putative DEAD‐box ATP‐dependent RNA helicase family protein [Zea mays] | 0.0 | FX986008 |
| R: GTTTCTTCCCATCTCGATCCCAAATCC | ||||||
| Cang_3069 | F: TGTGGAATTGTGAGCGGGTCTCCTCCGCCAAGTACTC | (AAG)10 | 398–439 | Poly(A)‐specific ribonuclease PARN [Ananas comosus] | 0.0 | FX986006 |
| R: GTTTCTTAATTGGAGGATGGCAAAGCG | ||||||
| Cang_3862 | F: CACGACGTTGTAAAACGACGATCCATCCACTCTCCCTCC | (AG)17 | 173–183 | Uncharacterized protein LOC100191912 [Zea mays] | 9.00E‐113 | FX986009 |
| R: GTTTCTTCATCCACCACGATACGCTTC | ||||||
| Cang_4293 | F: CTATAGGGCACGCGTGGTCGATTTCCACTGCGTGTACC | (AG)12 | 244–250 | PREDICTED: WD‐40 repeat‐containing protein MSI4‐like [Phoenix dactylifera] | 0.0 | FX986001 |
| R: GTTTCTTCCACTCGCAAACAACAGTCG | ||||||
| Cang_4398 | F: CGGAGAGCCGAGAGGTGTCCTACTAAAGTCCCTGCTGAG | (AG)12 | 147–153 | PREDICTED: uncharacterized protein LOC103721079 | 1.00E‐90 | FX985994 |
| R: GTTTCTTGTTGGTTGAGTGAGGCTGTG | ||||||
| Cang_5849 | F: CACGACGTTGTAAAACGACACCCACCCATAGTTCCAGAAG | (AG)10 | 406–418 | d‐cysteine desulfhydrase 2, mitochondrial‐like isoform X4 | 3.00E‐09 | FX986007 |
| R: GTTTCTTACCTATGAGTCAGCCCGAAC | ||||||
| Cang_7187 | F: CGGAGAGCCGAGAGGTGGCAGCGTGGGAAGGAAGAG | (AG)12 | 366–409 | WD repeat‐containing protein 44‐like [Ananas comosus] | 0.0 | FX986004 |
| R: GTTTCTTAAAGCGTTGGAAAGAGCGTC | ||||||
| Cang_7240 | F: CACGACGTTGTAAAACGACAAAGCTTGGCAGATTCGTCG | (AGG)11 | 210–218 | PREDICTED: protein TIC 21, chloroplastic [Musa acuminata] | 2.00E‐98 | FX985998 |
| R: GTTTCTTAATGCAGGCGTCGATGTTAC | ||||||
| Cang_7261 | F: CACGACGTTGTAAAACGACCTTCGTTTCACCACAGCTGC | (AG)12 | 236–238 | Protein ROOT PRIMORDIUM DEFECTIVE 1 [Asparagus officinalis] | 0.0 | FX986000 |
| R: GTTTCTTAAACCTCACCACTGCACTCG | ||||||
| Cang_10657 | F: CGGAGAGCCGAGAGGTGGAGGCGAATTGAGTTGCTCC | (AG)12 | 175–185 | Probable transcription factor At5g28040 [Ananas comosus] | 2.00E‐89 | FX985996 |
| R: GTTTCTTGCCAATGCCAAACTTTGAGG | ||||||
| Cang_18857 | F: CTATAGGGCACGCGTGGTCTCTCTCAGCTCGGACAGTG | (AG)12 | 398–408 | PREDICTED: protein TIFY 4B‐like isoform X4 [Phoenix dactylifera] | 8.00E‐58 | FX986005 |
| R: GTTTCTTTTCCACCGAAATCAGGGAGG | ||||||
| Cang_19507 | F: TGTGGAATTGTGAGCGGCACAGTATCTTTCTCCGCCC | (AG)19 | 201–215 | PREDICTED: histone deacetylase 19‐like [Elaeis guineensis] | 0.0 | FX986010 |
| R: GTTTCTTAGAAGTATGAGACCCGACGC | ||||||
| Cang_21384 | F: CACGACGTTGTAAAACGACGGGTTACCGAGGCACAATTG | (AC)12 | 118–136 | No significant similarity found. | — | FX985993 |
| R: GTTTCTTGATGCGACACAACTAACCCG | ||||||
| Cang_22899 | F: CTATAGGGCACGCGTGGTGGAGAGCAAATTCAGAGCGG | (AG)11 | 153–157 | PREDICTED: transcription factor PCL1‐like [Musa acuminata subsp. malaccensis] | 1.00E‐74 | FX985995 |
| R: GTTTCTTACAGAGAGAAGCAAGGCAGG | ||||||
| Cang_25819 | F: CTATAGGGCACGCGTGGTGGAGTTGATGATGGGTTTAGGG | (AG)17 | 287–297 | No significant similarity found. | — | FX986012 |
| R: GTTTCTTGGTCTGTGCCACTTAGTCCC | ||||||
| Cang_46532 | F: CGGAGAGCCGAGAGGTGAGCCCTAGAAACCTGACCTTG | (AC)12 | 302–305 | No significant similarity found. | — | FX986002 |
| R: GTTTCTTGGACACTATGCTGTACAAGGG | ||||||
| Cang_48335 | F: TGTGGAATTGTGAGCGGAGTTGTAGGTGGTGTAGCGG | (AG)10 | 185–189 | No significant similarity found. | — | FX985997 |
| R: GTTTCTTCCCTGGCACTGTTTAGCTTG |