Development and characterization of EST‐SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields

. 2018 Oct 19;6(10):e01185. doi: 10.1002/aps3.1185

Locus	Primer sequences (5′–3′)	Repeat motif	Allele size range (bp)	BLASTX top hit description	E‐value
Cang_103	F: CACGACGTTGTAAAACGACGATCGGTGATTGGGCCTTTG	(AG)₁₁	265	Glutamine synthetase root isozyme 3 [Zea mays]	0.0
	R: GTTTCTTGCCCTGATTTCTGAACCGTG			Glutamine synthetase root isozyme 3 [Zea mays]
Cang_594	F: CTATAGGGCACGCGTGGTTGCTCCAGTCCCAACCATAG	(AG)₂₀	325	PREDICTED: calmodulin‐binding transcription activator 4 isoform X1 [Elaeis guineensis]	0.0
	R: GTTTCTTTGGGTGTGCTTCTGAGACC
Cang_1002	F: TGTGGAATTGTGAGCGGCGGTGGTTGGAATTCGAAGG	(AG)₁₁	434	Sulfite exporter TauE/SafE family protein 4 [Sorghum bicolor]	4.00E‐143
	R: GTTTCTTTCCAGTTCACCTCCAGCTTC
Cang_1737	F: TGTGGAATTGTGAGCGGGAGAAATCAACAGAGCGGGC	(AAG)₁₄	414	PREDICTED: eukaryotic translation initiation factor 3 subunit I‐like [Phoenix dactylifera]	0.0
	R: GTTTCTTAACTGCGATTGGTCCTGTTG
Cang_1744	F: CACGACGTTGTAAAACGACTTCCTGGATCCTTGTCGACC	(AG)₂₀	276	PREDICTED: guanine nucleotide‐binding protein‐like NSN1 [Elaeis guineensis]	0.0
	R: GTTTCTTGCCTACATAACCCATCGCTC
Cang_2515	F: TGTGGAATTGTGAGCGGACCCTAGACTCGGATCCTCC	(AG)₂₃	279	Carbon catabolite repressor protein 4 homolog 1‐like [Ananas comosus]	0.00E+00
	R: GTTTCTTGCCAGACTTATACTCTCCCTCG
Cang_2955	F: CGGAGAGCCGAGAGGTGCTGTAACGAATCAGGTGCGG	(AAG)₁₀	410	Threonine dehydratase biosynthetic, chloroplastic [Ananas comosus]	0.0
	R: GTTTCTTCTCCATTACCTGCTCCCTCC
Cang_3156	F: CACGACGTTGTAAAACGACTTCAGTAGCCGAGCCTCATC	(AG)₁₂	413	Eukaryotic translation initiation factor 1A [Citrus clementina]	5.00E‐81
	R: GTTTCTTCCTCTCTTCCTGAACAAACCG
Cang_3166	F: CACGACGTTGTAAAACGACCGCTCTTGTGCAGTTCCAAC	(AT)₁₉	206	PREDICTED: uncharacterized protein LOC107807406 isoform X1 [Nicotiana tabacum]	2.00E‐177
	R: GTTTCTTGGGAGAGGGATCTGAGCTTG
Cang_3348	F: CTATAGGGCACGCGTGGTATTGCCTCCACAGCCTCC	(AG)₁₇	192	PREDICTED: NADP‐dependent d‐sorbitol‐6‐phosphate dehydrogenase [Elaeis guineensis]	0.0
	R: GTTTCTTAGCGGATAAGAGGAGATCGC
Cang_4013	F: TGTGGAATTGTGAGCGGACACGAAGCAGCTCTCTACC	(AG)₁₈	114	Peroxisome biogenesis protein 1 [Ananas comosus]	0.0
	R: GTTTCTTATTCGCCTCTGAGTCGAGAC			Peroxisome biogenesis protein 1 [Ananas comosus]
Cang_4089	F: CGGAGAGCCGAGAGGTGCACCTCCTCCTCTCTAAACCC	(AG)₂₄	198	Auxin response factor 18 [Ananas comosus]	0.0
	R: GTTTCTTCTGCTCTTCTCATTGGCGTC			Auxin response factor 18 [Ananas comosus]