Table 2.
Secondary structure characterization by circular dichroism spectroscopy
| A. nMOMP serovars extracted from adherent HeLa cells | |||||||
|---|---|---|---|---|---|---|---|
| Serovar | Serovar D,E,F, and J | ||||||
| Muridarum (%) | D (%) | E (%) | F (%) | J (%) | Mean (%) | SD (%) | |
| Helix | 11.6 | 19.0 | 22.0 | 20.3 | 19.0 | 20.1 | 1.4 |
| Antiparallel | 31.1 | 12.1 | 11.5 | 11.5 | 12.1 | 11.8 | 0.3 |
| Parallel | 5.8 | 12.1 | 11.2 | 11.8 | 12.1 | 11.8 | 0.4 |
| Beta‐turn | 17.3 | 17.6 | 17.7 | 17.3 | 17.6 | 17.6 | 0.1 |
| Rndm. coil | 34.1 | 39.2 | 37.5 | 39.0 | 39.2 | 38.7 | 0.8 |
| All β‐sheet | 36.9 | 24.2 | 22.8 | 23.3 | 24.2 | 23.6 | 0.7 |
| B. Chlamydia trachomatis Serovar E nMOMP extracted from various suspension cells | ||||||
|---|---|---|---|---|---|---|
| Suspension cell line | ||||||
| CAP (%) | CLDK (%) | CHO (%) | HeLa (%) | Mean (%) | SD (%) | |
| Helix | 29.6 | 27.3 | 23.7 | 28.1 | 27.2 | 2.5 |
| Antiparallel | 11.7 | 12.1 | 13.8 | 11.7 | 12.3 | 1.0 |
| Parallel | 9.1 | 9.6 | 10.2 | 9.4 | 9.6 | 0.4 |
| Beta‐turn | 17.5 | 17.3 | 17.3 | 17.2 | 17.3 | 0.1 |
| Rndm. coil | 32.1 | 33.8 | 35.0 | 33.6 | 33.6 | 1.2 |
| All β‐sheet | 20.8 | 21.7 | 24.0 | 21.1 | 21.9 | 1.5 |
Secondary structure composition of nMOMP calculated by deconvolution of circular dichroism spectra. (A) Chlamydia muridarum and C. trachomatis Serovars D, E, F, and J nMOMP obtained from adherent HeLa cells. (B) Chlamydia trachomatis Serovar E nMOMP obtained from infected suspension cell lines.