Figure 5.

Modular CHARGE system to detect various RNA targets. (a) Schematic of molecular beacon-based target detection. Here, the binding of target RNA induces the opening of the molecular beacon, which further activates the 1* domain of the original catalyst. (b) In vitro detection of miR21 (striped bars) and SgrS (white bars) with molecular beacon-incorporated D2 CHARGE. B: beacon, C: original catalyst target in D2, T: miR21 or SgrS target. (c) Confocal fluorescence imaging of cells expressing H1+H2+MBSgrs (SgrS- targeting molecular beacon) after adding different amounts of glucose and their cellular fluorescence intensity distributions. After 1.5 h IPTG induction, cells were exposed to glucose stress for 30 min. 200 μM DFHBI-1T was added for 30 min before imaging, and images were taken 45 min after adding DFHBI-1T. Here, individual cells were binned according to their brightness. The percentage of cells in each bin was plotted. A total of 200 cells were measured in each case from three experimental replicates.